ABSTRACT
In vivo Mammalian Bone Marrow Micronucleus Test is included in the standard battery genotoxicity testing,with great application prospects in medicine,public health,food and drug safety evaluation fields.Establishing standardized experimental methods and conditions in GLP condition and accumulating a certain range of background data could effectively ensure the reliability of the test system,and also provide strong basis to support the experimental data.We herein summarized the background data of mouse and rat bone marrow micronucleus tests performed from 2007 to 2015,to expound the standardized data collection method for rodent animal bone marrow micronucleus test.
ABSTRACT
Objective: To evaluate toxicological safety of Cordyceps sinensis and Panax quinquefolium compound according to the genetic toxicity study. Methods: Mice acute oral toxicity test, Salmonella typhimurium reverse mutation test, micronucleus test of mice bone marrow and mice sperm shape abnormality test were carried out in the compound. Results: The MTD of the compound was greater than 12.0 g/kg BW for both male and female mice in the acute oral toxicity test, which shows non-toxic substance. The reverse mutation number of Salmonella typhimurium reverse mutation test in five dose groups did not exceed 2-fold of the spontaneous revertant colony number, nor was there a dose-response relationship, the result of Ames test was negative. Micronucleus rate of each dose group for female mouse were 0.32%, 0.36%, and 0.40%, respectively. Micronucleus rate of each dose group for male mouse were 0.30%, 0.32%, and 0.40%, respectively. Sperm shape abnormality rate of each dose group were 2.4%, 2.3%, and 2.3%, respectively. Micronucleus rate and sperm shape abnormality rate had no significant increase compared with the negative control. The results of micronucleus test of mice bone marrow and mice sperm shape abnormality test were negative. Conclusion: Under this experimental condition, the genetic toxicity of the compound is not found, and it is classified as non-toxic.
ABSTRACT
The mouse (Mus musculus) bone marrow micronucleus test was carried out using 24 outbred National Institutes of Health (NIH) mice, 24 inbred Swiss Webster (CFW) mice and 20 inbred Bagg albino/color locus Jackson (BALB/cJ) mice. The mice in the experimental group (n = 32) were injected intraperitoneally with 133 mg kg-1 of metronidazole parenteral solution and the control group consisted of mice (n = 36) which had not been injected with metronidazole. There was no significant difference (p > 0.05) between the sexes regarding the micronucleus frequency in either the experimental or the control group. When the Mn frequencies of the three strains were compared, the results for the CFW and BALB/cJ strains did not differ statistically (p > 0.05) for either the experimental or control groups but there were significant (p < 0.05) differences between the CFW and NIH strains and the NIH and BALB/cJ strains for the experimental and control groups, with the NIH strain always showing the highest micronucleus frequency. Our results also show that metronidazole was possible genotoxic agent because it produced a significant increase (p < 0.05) in the micronucleus frequency of the experimental group as compared to the control group for all the three mouse strains tested.